National Mastitis Council
Laboratory Handbook on Bovine Mastitis
Table of Contents
Chapter 1 - Sample Collection and Handling
Introduction and General Information
Materials for Sampling
Sampling Technique
Sample Storage and Shipping
Chapter 2 - Diagnostic Equipment and Materials
Introduction and General Information
Tests
Commercial Microbiological Identification Systems
DNA Fingerprinting
Supplier Addresses
Chapter 3 - Diagnostic Procedures
Introduction and General Information
Primary Isolation
False-Positive and False-Negative Samples
Contaminated Samples
Chapter 4 - Streptococci
Introduction and General Information
Streptococcus agalactiae
Streptococcus dysgalactiae
Streptococcus uberis
Other Streptococcus species
Enterococcus species
Chapter 5 - Staphylococci
Introduction and General Information
Staphylococcus aureus
Coagulase-negative staphylococci
Chapter 6 - Gram-negative Bacteria
Introduction and General Information
Escherichia coli
Klebsiella species
Enterobacter species
Serratia species
Pseudomonas species
Proteus species
Pasteurella species
Chapter 7 - Miscellaneous Organisms
Introduction and General Information
Common Contaminants
Yeasts and Molds
Nocardia species
Prototheca species
Corynebacterium bovis
Arcanobacterium pyogenes (formerly Actinomyces pyogenes)
Mycobacterium species
Bacillus species and other Gram-positive bacilli
Mycoplasma species
Chapter 8 - Somatic Cell Counts
Introduction and General Information
California Mastitis Test
Wisconsin Mastitis Test
Direct Microscopic Somatic Cell Counts
Electronic Somatic Cell Counts
Chapter 9 - Bulk Tank Cultures
Introduction and General Information
BTM Culture Procedures
Results
Appendix 1 - General Isolation Media for Mastitis Causing Microorganisms
Blood Agar Plates
Blood-Esculin Agar Plates
Blood Agar Plates with Staphylococcus aureus Beta-Hemolysin
Preparation of Beta-Hemolysin
Appendix 2 - Mycoplasma Medium
Procedures
Ingredients for Mycoplasma Medium
Appendix 3 - Media
MacConkey Agar
Triple Sugar Iron Slants
Simmons Citrate Slants
Motility Test Medium
Camp-Esculin Plates
Carbohydrate Fermentation Media
Modified Rambach Agar Medium
Sabouraud Dextrose Agar Plates
Trypticase Soy Broth
6.5% NaCl Agar
Sodium Hippurate Medium
Potato Dextrose Agar
Prototheca Isolation Medium
Edwards Modified Medium
Vogel-Johnson Agar
Appendix 4 - Testing Procedures
CAMP-Esculin Test
Carbohydrate Fermentation
Esculin Hydrolysis
Sodium Hippurate test
Catalase Test
Oxidase Test
Coagulase Tube Test
KOH Test for Gram Staining Potential
MacConkey Agar Reaction
Triple Sugar Iron Agar
Simmons Citrate Agar
Motility
Growth in 6.5% NaCl
Appendix 5 - Stains
Methylene Blue Stain
Dienes Stain
Gram Stain
Acid-Fast Stain: Ziehl-Neelsen's Method
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